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What is melting temperature of DNA? Define denaturation and renaturation?

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MS SQL SERVER DBA Trainer

Denaturing/Renaturing a protein refers to a changing its conformation with respect to the "native conformation" of the protein (the one that exists en vivo). These conformational shifts can be induced by changing the salt concentration of the solution, adding/removing reagents or surfractants like SDS...
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Denaturing/Renaturing a protein refers to a changing its conformation with respect to the "native conformation" of the protein (the one that exists en vivo). These conformational shifts can be induced by changing the salt concentration of the solution, adding/removing reagents or surfractants like SDS or urea, or by heat. Just think, which one (denaturation or renaturation) refers to changing the protein's conformation away from its native state? -Hint: denaturation sometimes involves the breaking of disulfide bonds where Cys residues are present. read less
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Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be. If the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate...
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Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be. If the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. Denaturation is a process of formation of single stranded DNA from double stranded helical DNA upon heating. Renaturation is a process of formation of double stranded DNA or reannealing from single stranded DNA upon cooling. read less
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Biotechnologist

(Tm) the melting temperature is the temperature at which one-half of a particular DNA duplex which disassociate to single stranded DNA. Tm varies from species to species. Denaturation - formation of single stranded DNA from double stranded helix by heating. Renaturation - it is a process of formation...
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(Tm) the melting temperature is the temperature at which one-half of a particular DNA duplex which disassociate to single stranded DNA. Tm varies from species to species. Denaturation - formation of single stranded DNA from double stranded helix by heating. Renaturation - it is a process of formation of double stranded DNA from single stranded DNA by cooling. The concentration of DNA detected by measuring UV absorbanc at 260nm. read less
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Curriculam vitae

The melting temperature of DNA is called as Tm. The Tm is defined as the temperature in degrees Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands.
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Biologist

DNA denaturation, also called DNA melting denoted by Tm. The Tm is defined as the temperature in degress Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands. By using this formula you can calculate DNA melting temperature: ...
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DNA denaturation, also called DNA melting denoted by Tm. The Tm is defined as the temperature in degress Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands. By using this formula you can calculate DNA melting temperature: Tm = {2 (A+T) + 4 (G+C)} This takes into account that the G-C bond is stronger than the A-T bond. Note that the 2+4 rule is valid for a small length range only, about 20-40 nt. It is very easy to compute, but is of course very inaccurate. Where possible, it should be avoided. Denaturation: The process of formation of single stranded DNA from double stranded helical DNA upon heating that leads to breaking of hydrogen bonds between base pairs. Renaturation: The process of formation of double stranded DNA (reannealing) from single stranded DNA upon cooling. read less
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State level best teacher with R&D experience

DNA melting temperature is the temperature in degress Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands. And important point to understand here is, ‘melting’ in this sense is not a change of aggregate state, but...
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DNA melting temperature is the temperature in degress Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands. And important point to understand here is, ‘melting’ in this sense is not a change of aggregate state, but simply the dissociation of the two molecules of the DNA double helix. Factors which affect Tm are like, 1. Concentration of DNA 2. Concentration of ions in the solution, most notably Mg+ and K+ 3. DNA sequence 4. Length of DNA 50% GC always will not 50C, would be more than that, some time 70C also. Formulas used to calculate Tm are 2+4 rule of thumb (2* Number of AT + 4* Number of GC) its crude method used to estimate Tm of Primer with a max length of 40 nt, but its not accurate Linear regression method : Tm = 81.5 + 16.6(log10([Na+])) + .41*(%GC) – 600/length Here [Na+] is the molar sodium concentration, (%GC) is the GC ratio, and length is the length of the sequence. More well accepted method is, Nearest neighbour method which takes into account both the enthalpy of the pair formation between two nucleotides as well as the stacking effect between nearest neighboured nucleotide pairs. read less
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The melting temperature of DNA is called as Tm. The Tm is defined as the temperature in degrees Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands.
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resume new

When DNA is heated the double strands unwind and separate into single strands through the breaking of the hydrogen bonding between the bases. The term used to refer to this process is denaturation. Denaturation can be accomplished by other methods but in molecular diagnostic testing heat is the method...
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When DNA is heated the double strands unwind and separate into single strands through the breaking of the hydrogen bonding between the bases. The term used to refer to this process is denaturation. Denaturation can be accomplished by other methods but in molecular diagnostic testing heat is the method of choice. The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA. The higher the melting temperature the greater the guanine-cytosine (GC) content of the DNA. The melting temperature depends on both the length of the molecule, and the specific nucleotide sequence composition of that molecule. The guanine-cytosine pair is bound by three hydrogen bonds, while adenine-thymine pairs are bound by two hydrogen bonds. DNA with high GC content is more stable than DNA with low GC content. In PCR experiments GC content of primers are used to predict their annealing temperature to the template DNA. A higher GC content level indicates a higher melting temperature. Formula: Tm = 2 °C(A + T) + 4 °C(G + C) = °C Tm read less
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Ph.D Zoology ( Molecular biology)

The melting temperature (Tm) of the DNA means "the temperature at which the half of the Double Stranded DNA becomes Single Strand DNA" means this is the temperature at which the DNA is denaturating or melting from its Native form, hence we call that temperature as melting temperature of the DNA. Now...
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The melting temperature (Tm) of the DNA means "the temperature at which the half of the Double Stranded DNA becomes Single Strand DNA" means this is the temperature at which the DNA is denaturating or melting from its Native form, hence we call that temperature as melting temperature of the DNA. Now if DNA contains more GC base pairs( more hydrogen bonds comparitively than AT) then it requires more energy (temperature) for breaking the bonds. Here in this case the Tm of Primere varies depends on the composition. formula for predicting the Tm of the primer (4(G+C) + 2(A+T))-5. DNA denaturation refers to the unwinding and separation (melting) of the two strands of a double-helical DNA molecule.In DNA renaturation, double helical molecules are formed by the annealing of single-stranded molecules. DNA read less
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I believe in concept formation of students

For molecular biology applications such as PCR, sequencing or microarrays, it is important to determine the melting temperature of DNA, or Tm. The Tm is defined as the temperature in degrees Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are...
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For molecular biology applications such as PCR, sequencing or microarrays, it is important to determine the melting temperature of DNA, or Tm. The Tm is defined as the temperature in degrees Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands. Note that ‘melting’ in this sense is not a change of aggregate state, but simply the dissociation of the two molecules of the DNA double helix. read less
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